Gel Filtration Chromatography | Microbe Online
Gel Filtration Chromatography | Microbe Online
May 19, 2022
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SOLVED: A gel filtration column was calibrated by measuring elutionvolumes for two proteins of known mass:Glutamate dehydrogenase: 290 kDa withelution volume 20.8 mLSerum albumin: 37 kDa with elution volume42.5 mLUse these values to determine the equation of the straight linegraph that relates log molar mass to elution volume. The elution volume of an unknown protein was 22.7 ml. Use the equation just determined above to estimate the molarmass of the protein in kDa units.Please don't round your
SOLVED: A gel filtration column was calibrated by measuring elutionvo…
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