High-throughput RNAi libraries for mammalian cells are nearing a new maturity with the first wave of virally transfected short hairpin (sh)RNA libraries finally making it into published work.
The vector consists of a polyethylene glycolylated liposomal outer coat (providing immune evasion and prolonged circulation), a spindle-shaped nanomotor (resembling a flagellum, facilitating targeted ...
The EDMV vector was designed to express the large Cas9 protein and a sgRNA containing a customized spacer sequence. Infection of N. benthamiana and tobacco (N. tabacum) with the viral vector yield ...
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